Postnatal Gene Therapy Found Effective in SMA Mouse


Spinal muscular atrophy, a disease for which there is currently no effective treatment, might one day yield to gene replacement therapy. Two recent studies offer some intriguing insights into possible future directions of work in this field.

Early postnatal delivery of the gene for survival motor neuron 1 protein substantially increased survival rates and motor function in mice that were bred to display the spinal muscular atrophy.

Previous attempts to improve the long-term survival of mice that lack functional copies of the survival motor neuron 1 (SMN) gene have been unsuccessful, increasing survival only a few days to several weeks beyond the 15-day median life span of the mouse model of spinal muscular atrophy (SMA). But in two new studies, researchers increased the life span of the mice to beyond 250 days.

Both research groups used self-complementary adeno-associated virus vectors constructed with SMN (scAAV-SMN) to transduce lumbar spinal motor neurons and increase SMN protein levels in the brain and spinal cord, and—in the setting of intravenous administration—the muscles and vascular endothelium.

SMA in humans is caused by autosomal recessive inheritance of mutated SMN. “Although SMA children are often asymptomatic at birth, newborn screening that can detect SMA has been developed, supporting the feasibility of delivering SMN-scAAV9 to affected children,” Kevin D. Foust, Ph.D., of the Research Institute at Nationwide Children's Hospital, Columbus, Ohio, and his colleagues wrote in their report (Nat. Biotechnol. 2010 Feb. 28 [doi:10.1038/nbt.1610]).

They found that a single intravenous injection of scAAV9-SMN into 1-day-old SMA mice significantly improved their motor function and locomotive abilities. It also significantly improved their weight, but only to half that of control mice, likely because of incomplete transduction of all cells, wrote the researchers. Their work was funded with support from the Miracles for Madison Fund and the National Institute for Neurological Disorders and Stroke.

Aside from the mice that were euthanized for further analyses or died from an incidental cause, all lived beyond 250 days. By days 90-99, mice treated with scAAV9-SMN had fully restored neuromuscular transmission at neuromuscular junctions and a marked reduction in the accumulation of neurofilament at the junctions. If treatment was withheld until postnatal day 2, there was no difference in therapeutic effect, but if treatment was administered on postnatal day 5, survival was limited to 30 days. SMA mice that received treatment on postnatal day 10 were indistinguishable from those that did not receive scAAV9-SMN.

In a second study, Marco A. Passini, Ph.D., and his colleagues at the Genzyme Corp., Framingham, Mass., injected a similar SMN-integrated scAAV vector, scAAV8-SMN, into the cerebral lateral ventricles and the upper lumbar spinal cord of newborn SMA mice. They survived to a median of 157 days, gained weight, and were ambulatory throughout life (J. Clin. Invest. 2010 [doi:10.1172/JCI41615]).

At the age of 16 days, SMA mice treated with scAAV8-SMN expressed SMN at levels about 60%-90% of those seen in wild-type mice. At ages 58-66 and 120-220 days, the mice had SMN levels in the lumbar and thoracic segments that met or exceeded the levels in wild-type mice. At all time points, SMN levels were relatively low in the cervical spinal cord.

Improvements in the number of collapsed neuromuscular junctions were seen at 16 days of age in the quadriceps and intercostal muscles of SMA mice treated with scAAV8-SMN, but the number of these nonfunctional junctions later increased in mice that lived to 216-269 days.

Previous studies attempting treatment of SMA in mice have reported necrosis of the extremities and internal tissues. Dr. Passini and his colleagues observed a “very mild” necrosis of the distal hind legs after treatment that did not require euthanasia. Most (60%) mice in that study were sacrificed because of a sudden appearance of respiratory distress.

Dr. Foust and his coauthors reported only vascular necrosis of the pinna after treatment with scAAV9-SMN.

Dr. Smith's comment: Gene replacement therapy is the holy grail of a number of life-threatening inherited neurological diseases. Inroads on new treatments have been made for Duchenne's muscular dystrophy and Pompe's disease, but not for hereditary anterior horn cell diseases such as the autosomal recessive SMAs.

Attempts to improve muscle function and increase the expression of survival motor neuron 1 (SMN1) using inhibitors of histone deacetylase have yielded only modest results in human subjects with SMA types III or IV. However, in the current studies, researchers were able to increase motor function and survival rates substantially.

The promising results obtained by Dr. Foust's and Dr. Passini's research teams are an early step in the long road of seeking to establish gene replacement therapy as a viable treatment in patients with SMA. Even so, they are a ray of hope on what has been a very dark horizon for families and their loved ones diagnosed with one of these serious disorders. The field anxiously awaits (with appropriately cautious optimism) publication of additional efforts to take gene replacement therapy of SMA (and perhaps in time other inherited motor neuronopathies) closer to the goal of efficacious treatment.

Next Article: