Article

Long-term function of canine autograft kidneys that had been preserved extracorporeally for twenty-four hours

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Abstract

SUCCESSFUL short-term preservation of canine kidneys has been reported by several authors1–3 in recent years, with data relating to the immediate functional and pathologic status of the preserved kidneys. Bauditz and associates4 from our laboratory reported the immediate results of 24-hour preservation of canine kidneys by means of hypothermic pulsatile perfusion of treated homologous plasma and a film oxygenator. Our report presents the results of functional and pathologic studies of kidneys of six mongrel dogs that were killed after surviving for from 306 to 376 days (an average of 338.5 days) each with a solitary kidney autograft previously maintained extracorporeally for 24 hours by that method of preservation.

Methods

A series of eight dogs comprised the long-term study. All dogs were housed on a farm and fed a diet consisting of meat and Ken-L-Ration dog food. The renal function of each dog was maintained by a solitary kidney. One kidney of each of six dogs had been extracorporeally preserved for 24 hours, then reimplanted as an autograft, with a contralateral nephrectomy performed at the same time. The other two dogs served as controls. In each control animal, the solitary kidney was simply flushed with heparinized saline and autotransplanted to the iliac fossa, with an immediate contralateral nephrectomy performed at the same operation. When killed, the two control dogs had survived 420 days, and 398 days.

The perfusate used for preservation of the kidneys was prepared in the following manner. Pooled commercial dog plasma was kept frozen until time of . . .


 

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