Article

Commercial Glucose Oxidase Preparations for the Detection of Glucose in Urine

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Abstract

ALTHOUGH enzymes which oxidize glucose are abundant in nature and have been known for many years, until recently, no application of their activity has been made in clinical biochemistry. The first enzymatic preparation with specific glucose oxidase activity was described by Müller1 in 1928. Subsequent workers have isolated other preparations. The best known of these enzyme preparations is notatin, which Coulthard and associates2 isolated from Penkillium notatum and described in detail in 1945. These enzymes are flavo-proteins, and they catalyze the aerobic oxidation of glucose to gluconic acid with the production of hydrogen peroxide.

Recently there have appeared on the market two commercially prepared glucose oxidase reagents, Clinistix* and Tes-Tape,** which are designed to detect the presence of glucose in urine by color changes of reagent-treated paper. Each of these preparations is specific for glucose and no heating is required to bring about the reaction. This fresh approach to the detection of glucose after more than a century of exploitation of copper reduction by glucose seemed to warrant clinical trial of these two new preparations. Our report presents the results of a clinical trial, and evaluates both the new preparations and the conventional solution with respect to sensitivity, specificity, convenience, and economy.

Materials and Methods

The standard Benedict's technic used in our laboratory involves the use of Benedict's solution prepared from precompounded Benedict's reagent. Five milliliters of reagent and 5 drops of urine are mixed and boiled for 5 minutes in a water bath. At the end of that time,. . .


 

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