Immunoglobulin and T-cell receptor gene rearrangement
Andrew J. Fishleder, MDAddress reprint requests to A.J.F., The Cleveland Clinic Foundation, 9500 Euclid Avenue, One Clinic Center, Cleveland, Ohio 44195.
Recent studies have significantly broadened understanding of immunoglobulin production and T-cell receptor formation at the gene level. A limited number of genes can be rearranged during the course of B-cell or T-cell development to yield unique DNA sequences that will code for specific antibodies and T-cell receptor proteins. Southern blot hybridization analysis allows sensitive examination of lymphocyte DNA for the presence or absence of gene rearrangements. General mechanisms that underlie immunoglobulin and T-cell receptor rearrangement are reviewed, along with the diagnostic applications of detection of gene rearrangement by Southern blot hybridization technique.