Experimental preservation of sheep kidney for successful transplantation

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ALTHOUGH it is now possible to preserve isolated mammalian kidneys for 24 hours or longer, the functional potentiality of the organ so preserved is greatly reduced. At the present stage of human cadaver kidney transplantation, it is important to know the safe maximal period of ischemia which will permit adequate function of the organ to sustain life after transplantation. With evidence of the importance of histocompatibility in regard to the future fate of the transplanted cadaver kidney, it is urgent to define a safe period of ischemia which will allow such matching tests as are currently available.

This report presents the data of our experimental study of the effects of different periods of ischemia on the degree of irreversible renal damage and recovery of function after autotransplantation of kidneys in sheep.

Kidneys were preserved with hypothermia and hyperbaric oxygen and were evaluated after autotransplantation in the necks of the animals. Thus, the effects of preservation were studied without interference from complications induced by immunologic reactions. Lack of laboratory space prevented long-term evaluation of the results, but even at short term the differences in renal function indicated that one method was superior.

Materials and Methods

Thirty sheep kidneys were excised and were preserved as follows. Twenty-one sheep kidneys were preserved by a combination of hypothermia at 2 C and hyperbaric oxygen at a pressure of 30 psi (3 atm absolute) from 10 sheep, and of 60 psi (6 atm absolute) from 11 sheep. Nine kidneys were preserved by hypothermia alone at . . .



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