Inhibition of Growth of Mouse Tumors by Injections of Serotonin or serotonin and Histamine Combined

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IN a previous publication1 it was noted that daily injections of serotonin creatinine sulfate into tumors of mice “cured” 7 of 55 B6D/2F1 hybrid mice with S91 melanomas implanted on their feet, and caused striking inhibition of the growth of the tumors in all treated mice. Pukhalskaya2 also has observed antitumor effects of serotonin, noting that serotonin hydrochloride caused a significant inhibition in a number of animal tumors as well as an inhibition of cell division in in vivo experiments on the regenerating tadpole tail and rat cornea. Pukhalskaya stated that serotonin must be used in the form of the hydrochloride rather than the creatinine sulfate salt, and emphasized that it must be injected hypodermically and not intraperitoneally. The dosage employed was 20 mg. per kilogram of body weight per day for eight days.

Additional observations on the regression of mouse tumors after injections of serotonin are presented in the following report.

Materials and Methods

Four types of tumors were minced and transplanted to the webs of the left hind feet of five types of mice as follows:

  1. S91 melanoma in DBA1 or B6D/2F1 hybrid mice, male or female, 6 weeks old.

  2. Sarcoma 180 in female Swiss mice, 6 weeks old.

  3. T241 sarcoma in male or female C57BL/6 mice or in B6D/2F1 hybrids, 6 weeks old.

  4. Sarcoma 1 in strain A mice, 6 weeks old.

The natural history of these tumors has been discussed in detail elsewhere.1 Sarcoma 1 grows rapidly when implanted on the hind. . .



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