Electrophoretic and Ultracentrifugal Analysis of Serum Proteins in Multiple Myeloma*

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WHILE many studies have been made on the serum proteins in multiple myeloma, the application of both electrophoretic and ultracentrifugal analysis to the same sera has been limited. This report summarizes the results of electrophoretic and ultracentrifugal analyses of the serum or plasma proteins of 10 patients with multiple myeloma. Seventeen additional cases had electrophoretic analyses of the sera.

Electrophoresis was carried out by the method of Tiselius as modified by Longsworth,1 using either phosphate buffer pH 7.8, ionic strength 0.16 μ, or barbiturate buffer, pH 8.6, ionic strength 0.1 μ. Ultracentrifuge studies were made in a cell holding 0.9 ml. of solution; a speed of 59.780 r. p. m. exerting a force equivalent to 254.500 times gravity was employed. The serum was dialyzed against 0.9 per cent sodium chloride, and the protein concentration adjusted to approximately 1 per cent, before centrifugation.

The diagnosis of multiple myeloma was, in all cases, established by blood and bone marrow studies and physical examination. Clinical notes on 4 of the cases are included because of unusual findings in the protein studies or difficulty in the establishment of diagnosis.


The electrophoretic serum protein pattern in multiple myeloma can be divided into four groups (table 1). In t he first, which represented 7 of the 27 cases, there was a definite increase in the serum protein component with mobility similar to that of normal β-globulin. One serum showed only a slightly elevated β-globulin concentration, but 90 per cent of the urinary protein had a. . .



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